As Melania was preparing to address the applauding crowd, the president crossed the stage behind her, grasping her upper arm in the process.
His action caused her to flinch, turn her head downward and take a sharp breath in.
The first lady appeared to need a moment to regain her composure before continuing on and leading the crowd in the Lord's prayer.
See her reaction at 0:11:
— Fox News (@FoxNews) February 18, 2017
People took to Twitter to share their concerns for the first lady, saying her body language was consistent with that of a domestic violence victim.
Check out reactions on Twitter:
This isn't the first time concerns have been raised over Trump and Melania's relationship.
A video from the Presidential Inauguration sparked the hashtag #FreeMelania last month.
In the short clip, Melania can be seen smiling at her husband -- but the moment he looks away, her smile disappears into a much more somber scowl.
A photo of the first lady watching the Super Bowl at Trump's Mar-a-Lago golf club while sporting a similar expression reignited the hashtag on February 5.