Christianity Today editor in chief Mark Galli published the editorial on Thursday that said the facts revealed in the House impeachment process were "unambiguous."
"That he should be removed, we believe, is not a matter of partisan loyalties but loyalty to the Creator of the Ten Commandments," Galli wrote.
Trump took to Twitter to take down the publication, saying the "far left" magazine has been "doing poorly."
"A far-left magazine, or very 'progressive,' as some would call it, which has been doing poorly and hasn't been involved with the Billy Graham family for many years, Christianity Today, knows nothing about reading a perfect transcript of a routine phone call and would rather ... have a Radical Left nonbeliever, who wants to take your religion & your guns, than Donald Trump as your President," he wrote on Twitter.
The president also claimed that no other president has "done more for the Evangelical community."
"It’s not even close," he continued. "You’ll not get anything from those Dems on stage."
Galli, who told CNN on Friday he is leaving the publication, said Trump's characterization of the magazine as "far left" is "far from accurate."
The editorial came as an unexpected move from the magazine, founded by Billy Graham in 1956. The magazine usually avoids weighing in on the political conversation but has criticized Trump before on some issues. It had not, however, previously called for his removal.
Franklin Graham, founder Billy Graham's son, responded to the op-ed on Twitter and claimed that his father would not agree with the ideas presented; instead, he would "be disappointed," he said.
"My father knew @realDonaldTrump, believed in him & voted for him. He believed Donald J. Trump was the man for this hour in history for our nation," Graham tweeted.
Jim Wallis, one of the nation's most prominent liberal evangelical leaders, told CNN the op-ed, which generated so much traffic it caused issues on web servers, may have created a rift in evangelical support for Trump.
"You don't need a whole lot to shift the race, just a few votes in key places in key states could make the ultimate difference," Wallis said.