Second suspect in murder of 8 family members on Ohio pot farm pleads not guilty

A second suspect in a mass killing of eight members of one family on an Ohio marijuana farm pleaded not guilty to eight counts of aggravated murder, among other charges, in court on Wednesday.

George Wagner IV, 27, pleaded not guilty to a total of 22 counts, including the eight counts of aggravated murder with death penalty specifications, in Pike County court in Ohio. A judge denied Wagner's request to be released on bail.

Wagner is accused of helping his brother and parents kill the mother of his niece and seven other members of the Rhoden family on April 22, 2016.

On Tuesday, George Wagner IV's brother, Edward "Jake" Wagner, 26, was the first of the family to be arraigned. He pleaded not guilty to 23 counts.

Related: Legal recreational marijuana sold in California

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Legal recreational marijuana sold in California
Customers buy recreational marijuana at the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
Marijuana is displayed for sale at the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
A customer browses marijuana products for sale at the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
Customers queue for recreational marijuana outside the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
A customer browses screens displaying recreational marijuana products for sale at the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
A woman holds marijuana for sale at the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
Marijuana edibles are displayed for sale at the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
Eron Silverstein, 51, (R) shops for marijuana at the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
Marijuana products are displayed for sale at the MedMen store in West Hollywood, California U.S. January 2, 2018. REUTERS/Lucy Nicholson
Customers purchase marijuana at Harborside, one of California's largest and oldest dispensary dispensaries of medical marijuana, on the first day of legalized recreational marijuana sales in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
People wait in line at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana sales in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
A customer waits at the counter to purchase marijuana as others wait in line at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
Andrew DeAngelo (L) and his brother Steve DeAngelo (R), co-founders of Harborside, one of California's largest and oldest dispensaries of medical marijuana, celebrate after a ceremonial ribbon cutting on the first day of legalized recreational marijuana in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
An employee hugs a customer as others wait in line at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
An employee finds marijuana for a customer at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
Employees wait behind the counter at Harborside, one of California's largest and oldest dispensaries of medical marijuana, as a large clock counts down to the store's official opening at 6am on the first day of legalized recreational marijuana in Oakland, California, U.S. January 1, 2018. REUTERS/Elijah Nouvelage
Different strains of marijuana are seen for sale at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
A couple poses behind a cardboard Instagram frame while waiting in line at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
Employees prepare to open at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
Steve DeAngelo (C) makes the first legal recreational marijuana sale to Henry Wykowski at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana sales in Oakland, California, U.S. January 1, 2018. REUTERS/Elijah Nouvelage
Michael Sherman purchases marijuana at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana sales in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
A customer peers at different marijuana strains in a glass case at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
Marijuana is seen for sale at Harborside, one of California's largest and oldest dispensaries of medical marijuana, on the first day of legalized recreational marijuana sales in Oakland, California, U.S., January 1, 2018. REUTERS/Elijah Nouvelage
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A custody dispute between Edward "Jake" Wagner and his former girlfriend, Hanna Rhoden, is believed to be the motive behind the killings, according to authorities. Among the Rhoden family members killed was a teenager.

Edward "Jake" Wagner's charges include an additional charge of unlawful sexual conduct with a minor due to his relationship with Hanna Rhoden when she was 15 and he was 20.

The family patriarch, George Wagner, is due to be arraigned on Dec. 4. Angela Wagner, the mother of the suspects who have already been arraigned, will go before a judge on Thursday.

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