The 18-minute video shows photographs one-by-one of the 49 victims who were gunned down less than three weeks ago at the Orlando nightclub, Pulse. A different celebrity narrates each picture by describing the victim's life.
The Human Rights Campaign released the video which is directed by Murphy and Ned Martel. The project was filmed at Fox Studios in Los Angeles over the course of three days.
"The hate that stole these 49 individuals from all those who loved them has been allowed to flourish in our country for too long," Murphy said in a statement. "No person is born hating another, and we have an obligation as Americans to stand up against prejudice and bigotry that would incite violence against someone simply for who they are."
A full list of the participants is: Lady Gaga, Chris Pine, Cuba Gooding Jr., Connie Britton, Matt Bomer, Sarah Paulson, Angela Bassett, Lea Michele, Colton Haynes, Sophia Bush, Jane Fonda, Harry Shum Jr., Denis O'Hare, Rob Reiner, Melissa Benoist, Caitlyn Jenner, Édgar Ramírez, Max Greenfield, Chaz Bono, Cheyenne Jackson, Emma Roberts, Kerry Washington, George Lopez, Evan Rachel Wood, Sofia Vergara, Diego Boneta, Nina Jacobson, Demi Lovato, Tyler Oakley, Yeardley Smith, Kid Cudi, Kaitlin Olson, Kevin McHale, Jamie Lee Curtis, Lee Daniels, Chace Crawford, Evan Peters, Gerard Butler, Katey Sagal, John Stamos, Laverne Cox, Jordana Brewster, Wes Bentley, Finn Wittrock, Darren Criss, Kathy Bates, Anna Paquin, Guillermo Díaz and Joe Mantello.
See photos of the Orlando victims: